SPHERIOGRAPH approach presents a dynamic column-based method for the evaluation and imaging of radiotracer in a 3D cell culture environment. More precisely, this highly interdisciplinary project comprises

-          development and evaluation of an in vitro column-based 3D cell culture methodology, utilizing co-culture systems with diverse cells expressing different targets mimicking a real tissue situation.

-          manufacturing and optimization of the column scaffold (stationary phase), allowing the controlled application of the radiotracer at a defined speed (mobile phase) which guarantees equal distribution of the applied radiation dose and nutrients, CO₂/O₂ supply, etc. required for viable cells.

-          online monitoring of vital cell culture parameters (pH, nutrient, and CO₂/O₂ supply among others) by sensors or specific tracers ([¹⁵O]H₂O, [¹¹C]CO₂, among others).

-          validation and assessment of this new in vitro tool using metabolic radiotracers (e.g. [¹⁸F]FDG, [¹⁸F]FET, [¹¹C]methionine), radiotracers for receptor targeting (e.g. [⁶⁸Ga]Ga-PSMA, [¹¹C]DASB, [¹¹C]metoclopramide), or others ([¹⁸F]FEC, [¹⁸F]FMISO).